Photography

Photomicrographs were obtained using a color filter system in a Zeiss microscope with an $40 \times$ PLANAPO oil immersion objective and a $10 \times$ ocular. A total of 85 photographs represented about $98 \%$ of the entire nerve. Images were then digitized by a system Nikon 25-1000 software with a size of $1850 \times 1234$ pixels. Each photograph was enlarged by the digitizing process ( $\times 3.3$) to give a final magnification of $\times 1695$. A microscale (0.01 mm, Wild, Switzerland) was processed in the same way for scaling. The pixel size was found to be $0.1135 \mu m$.

  

Figure 4.3: Part of a typical image in the nerve cross-section after tissue preparation, staining and digitization